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HomeEventsOSSC January Meeting - Dr. Tijana Jovanovic-Talisman - Superresolution Microscopy

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OSSC January Meeting - Dr. Tijana Jovanovic-Talisman - Superresolution Microscopy

When:
Wednesday, January 13, 2016
Where:
Kellogg West Hotel and Conference Center On the Cal Poly Pomona Campus
Kellogg West Hotel and Conference Center
Horse Hill Drive (off of S. University Drive)
Pomona, CA  91768
Additional Info:
Event Contact(s):
Bo Wang
Category:
OSSC Regular Meeting
Registration is closed
Payment in Full In Advance Or At Event
Cancellation Policy:
Please let the Arrangements Chair (Events@ossc.org) know as soon as possible.

Payments made in advance will normally be issued a credit that may be applied to future payments (membership or events). Please contact the Treasurer (Treasurer@ossc.org) if this posses a significant burden.
Activities/Items    (Click the down-arrow to the left of the activity/item to view the details)
Meeting & Presentation
Dinner
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Advances in Quantitative Super-Resolution Microscopy

Dr. Tijana Jovanovic-Talisman

Assistant Professor of Molecular Medicine, City of Hope

2016 January image-superres 

Hosted by Cal Poly Pomona

Abstract: Significant advances in super-resolution microscopy include recently developed methods for quantitative data analysis. While these are powerful tools for evaluating the lateral distribution of proteins, the analysis process is still challenging because quantification requires the biophysical properties of the super-resolution probes to be accurately defined. This is a time intensive process that has to be completed for each fluorophore, set of acquisition parameters, and microscope platform. To streamline the process, we designed a novel and efficient in vitro assay for characterizing super-resolution probes. First, we established robust chemistry to covalently immobilize proteins to a glass surface with minimal background signal. Using this approach, we evaluated photoactivatable proteins (pa-GFP and pa-mCherry1) and fluorescent molecules (ATTO 488 and Alexa 647). The biophysical properties that we uncovered for the fluorophores were then used to quantitatively investigate organization of proteins on the cell plasma membrane.
Reception: 6:00; Dinner: 7:00; Talk: 8:00
Meal $35 for registration by January 7, $40 for registration after
Free for OSSC student members who register by January 7, $15 for student members who register after



Please Register by Thursday, January 7, 2016
 

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